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Detection of Biomarkers for Periodontal Diseases Using Salivary Proteomics
김현철, 김형섭, 문경숙, 조남표,
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김현철 ( Kim Hyun-Chul ) - 전북대학교 치의학전문대학원 치주과학교실
김형섭 ( Kim Hyung-Seop ) - 전북대학교 치의학전문대학원 치주과학교실
문경숙 ( Moon Kyung-Suk ) - 전북대학교 치의학전문대학원 구강병리학교실
조남표 ( Cho Nam-Pyo ) - 전북대학교 치의학전문대학원 구강병리학교실
KMID : 0829220100340030119
Abstract
Because of the irreversible nature of periodontal disease, early diagnosis is an important aspect of management of patients with periodontal disease. Human saliva is an attractive medium for disease diagnosis because its collection is noninvasive and simple. Analysis of saliva may be especially beneficial in the determination of current periodontal status and serve as means for the screening of periodontal disease. In the present study, we investigated potential biochemical markers in whole saliva samples for the screening of periodontal disease using proteomics technique. We enrolled five subjects each from four different groups on the basis of measures of periodontal health (healthy group, gingivitis group, chronic periodontitis group and aggressive periodontitis group). Eleven proteins in whole saliva samples were identified as differentially expressed proteins between the healthy and periodontal disease groups using 2-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight / time-of-flight mass spectrophotometry (MADLI-TOF/TOF MS) approaches. Although the diagnostic value of oral fluid has been recognized for some time and potential biomarkers of periodontal disease have been identified in saliva, this, to our knowledge, is one of the first studies to examine large-scale proteomic profiling to identify the extent of periodontal destruction. Thus, this work provides an important framework for future efforts aimed at understanding salivary responses to periodontal destruction and predicting the future disease progression.
키워드
Periodontal disease; Saliva; Biomarker; Proteomics
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